北京生物医学工程

高血压大鼠视网膜血瘀出血图像采集与处理

Image acquisition and processing of retinal bloodstasis in hypertensive rats

作者：张魁星张方申彭欣

单位：山东中医药大学理工学院(济南250355)

分类号：R318.04

出版年·卷·期（页码）：2017·36·5（441-445）

摘要：

目的研究高血压大鼠视网膜眼底图像的采集与分析方法，以应用于血瘀出血机制研究。方法实验分2组，对照组为20只健康雄性Wistar大鼠，模型组为20只雄性高血压大鼠(SBP≥140mmHg)，利用视网膜照相机及微循环显微镜采集视网膜图像；运用图像处理技术计算动静脉管径比(DA/DV)、出血面积等形态学参数等。结果模型组大鼠视网膜微血管数量减少,DA/DV 明显减小,血液流速减慢,与对照组相比，差异均具有统计学意义(P<0.05)。高血压大鼠视网膜图像存在着明显的瘀血出血现象；经图像处理后，此病理变化的图像特征可以得到清晰的量化表达。结论从微血管形态学角度，明确了高血压模型存在着血瘀出血的病理改变；同时，也为高血压血管损害与治疗效果的评判，提供了深入研究的有效方法及客观评价标准。

Objective To study the preparation and quantitative evaluation criteria of blood stasis image of hypertensive rats. Methods The rats were divided into the model group (20 male hypertensive rats, SBP≥140mmHg) and the control group (20 male healthy Wistar rats).Retinal camera and mesenteric microcirculation observation system were used to perform the observation.The image processing technique was used to calculate the morphological parameters,such as the diameter ratio of artery to vein and the area of bleeding. Results The number of the retinal microvascular in the model groupdecreased,DA/DV decreased, blood flow reduced, compared with the control group,the differences were statistically significant (P<0.05). Retinal images of hypertensive rats had obvious blood stasis and bleeding phenomenon; after processing,image characteristics of the pathological changes could get a clear quantitative expression. Conclusions From the perspective of microvascular morphology,there is a clear pathological changes of blood stasis model of hypertension hemorrhage. At the same time, for blood vessels and therapeutic effect evaluation,it also provides some effective methods for further research and objective evaluation criteria.

参考文献：

［1］吕干,尹瑞兴.高血压用药干预的研究进展［J］.中国临床新医学，2016,9(11):1053-1056.

Lyu G,Yin RX.The research progress of antihypertensive drugs［J］.Chinese Journal of New Clinical Medicine,2016,9(11):1053-1056.

［2］张晨，王宝和.高血压眼底病变的中医药治疗进展［J］.天津中医药，2016,33(7)：445-448.

Zhang C,Wang BH.Recent advances in thetreatment of hypertension retinopathy of traditional Chinese medicine［J］.Tianjin Journal of Traditional Chinese Medicine,2016,33(7)：445-448.

［3］赵惠淳，张方申，张海燕，等.自发性高血压大鼠微循环障碍与血瘀、出血的相关性研究［J］.山东中医杂志，2016,35(11):985-988,995.

Zhao HC，Zhang FS，Zhang HY,et al.Studyon blood stasis and bleeding correlated with microcirculation disturbance in spontaneously hypertensive rats［J］.Shandong Journal of Traditional Chinese Medicine,2016,35(11):985-988,995.

［4］张海燕,张方申,秦林,等.血瘀出血兼证的现代临床诊治研究概况［J］.现代中西医结合杂志，2015,24(25)：2842-2845.

［5］温艳东,易亮,范铁兵.化瘀止血散治疗上消化道出血的临床疗效观察［J］.中国中医基础医学杂志，2015，21(8)：974-976.

［6］刘文舟.眼底视网膜作为最佳窗口对全身微小血管病变的诊断和预测［J］.宁夏医学杂志，2015，37(4)：381-384.

［7］黄雯.重视视网膜血管异常性疾病与慢性肾脏病的整合医学研究［J］.中华眼科医学杂志，2015,5(6)：280-290.

Huang W.Emphasis on the integration of retinal vascular abnormalities and chronic kidney disease［J］.Chinese Journal of Ophthalmologic Medicine,2015,5(6):280-290.

［8］Winder RJ,Morrow PJ,McRitchie IN,et al. Algorithms fordigital image processing in diabetic retinopathy［J］. ComputerMedical Imaging and Graphics,2009,33(8):608-622.

［9］朱承樟,邹北骥,向遥,等.彩色眼底图像视网膜血管分割方法研究进展［J］.计算机辅助设计与图形学学报,2015,27(11):2046-2056.

Zhu CZ,Zou BJ,Xiang Y,et al.A survey of retinal vessel segmentation in fundus images［J］.Journal of Computer-Aided Design & Computer Graphics,2015,27(11):2046-2056.

［10］王红星,韩倩,张琰,等.眼底像血管管径测量与眼病［J］.眼科新进展,2014,34(2):197-200.

Wang HX,HanQ,Zhang Y,et al.Measurement of fundus vessel caliber and eye diseases［J］.Recent Advance in Ophthalmology,2014,34(2):197-200.

［11］张方申,彭欣,秦林,等. 痰瘀互结型动脉粥样硬化大鼠视网膜和肠系膜微循环观察［J］.微循环学杂志,2015,25(1):10-13,22.

Zhang FS,Peng X,Qin L,et al.Microcirculation of the retina and mesenteric in atherosclerosis rats with syndrome of intermingled phlegm and blood stasis［J］.Chinese Journal of Microculation,2015,25(1):10-13,22.

［12］王小俊,刘旭敏,关永.基于改进Canny算子的图像边缘检测算法［J］.计算机工程,2012,38(14):196-198.

Wang XJ,Liu XM,Guan Y.Image edge detection algorithm based on improved Canny operator［J］.Computer Engineerin,2012,38(14):196-198.

［13］李伟,彭欣,王冰,等.四物汤调节血虚大鼠微循环机制的实验研究［J］.陕西中医,2009,30(12):1662-1663.

［14］黄晓燕，陈碧新，高瞻,等.自发性高血压大鼠视网膜微血管稀少与细胞凋亡［J］.温州医学院学报，2010,40(2):111-114.

Huang XY,Chen BX,Gao Z,et al.Apoptosis and microvascular rarefaction of the retinal in spontaneously hypertensive rats［J］.Journal of Wenzhou Medical College,2010,40(2):111-114.

服务与反馈：

【文章下载】【加入收藏】

提示：您还未登录，请登录！点此登录