Objective By combining gait features with physical condition, this paper aims to analyze and compare the differences and rules of natural gait feature among college students with different physical condition. The physical condition of college students will be reflected by the gait features, which provides certain theoretical basis for improving the physical condition of undergraduates. Methods One hundred and nineteen undergraduates were selected as research subjects and their physical condition were tested and thus classified according to National Fitness and Health Standard of Students (revised in 2014) . The natural trait features of classified students were tested through the test system of plantar pressure. The latent parameters included the percentage of different foot support periods, the peak value of pressure among different pelma areas, the percentage of pulse received by different pelma areas, arch index and axial angle of the foot. Finally, the differences of gait features were compared and analyzed.Results There was a significant difference in the size of axial angles among subjects walking naturally.Conclusions The physical condition is closely related tothe gait feature of undergraduates. The physical condition of undergraduates can be further understood on the basis of differences in gait features, which can provide objective evidence aiming at devising undergraduateoriented physical training scheme and thus promoting physical condition of undergraduates.