Objective In order to manage and evaluate the teaching effectiveness of total English teaching in medical physiology curriculum, we establish a curriculum management module and online examination database based on SQL Server 2008 platform.By the function of data editing, inquiry, extraction and the data security guarantee mechanism of the platform, an assessment system is developed including curriculum management, online test question management, test scoremanagement and statistical analysis. It can investigate the learning effects of Chinese and foreign students of medicinae baccalaureus, baccalaureus chirurgiae (MBBS) class with multiple factors of language background and educational background. Methods Using curriculum test management module to evaluate the effectiveness of total English teaching in medical physiology curriculum carried out in the Chinese and foreign students of2012 and 2013 MBBS class. In order to understand how students from different culture background master the key knowledge points in the important chapters, their final scores are collected and analyzed. The formation of outstanding students and the possibility in continually development in the professional filed are concluded through statistical analysis. Results The data shows that MBBS students from different culture background and owned different language backgrounds mastering key knowledge in total English teaching physiology courses have statistically significant difference (P<0.05) . Foreign students with English education background and the education above high school level have great advantages in studying medical physiology. Conclusions The assessment system based on SQL Server platform, with formative assessment score statistic result feedback mechanism and final assessment score analysis, is a good analysis system to investigate the process of high quality students growing. It is of great help to objectively evaluate the formation process of clinical undergraduate students with different language, culture and education background.