北京生物医学工程

一种具监测功能的高精度微量移液器的设计

Design of a high-precision micropipette with monitoring function

作者：张晓亮王弼陡罗刚银

单位：中国科学技术大学生物医学工程学院（合肥 230026）中国科学院苏州生物医学工程技术研究所（江苏苏州 215163）

关键词：检验仪器；微量移液； PID算法； STM32；液面探测

分类号：R318.6; TH715.3

出版年·卷·期（页码）：2019·38·4（407-416）

摘要：

目的针对全自动临床检验仪器传统移液方式的精度以及可靠性限制问题，设计一款带有液面监测功能的高精度微量移液器。方法基于气动置换技术，以STM32微处理器为主控芯片，通过实时检测移液器内部压力变化，实现了液面探测、吸样异常监测等功能。采用位置-速度双闭环PID算法实现对直线步进电机的精确定位，提高样本吸取的准确度。最后，通过移液过程监测实验和吸样精度验证实验，验证了设计的微量移液器的液面探测功能、吸样异常识别功能及吸样精度。结果该微量移液器移液精度较高，移液误差较小，重复性较好，并能实现移液的全过程监测。结论本文设计的微量移液器满足JJG 646—2006国家标准设计规范，适用于全自动临床医疗检验仪器。

Objective A high-precision micropipette with monitoring function was designed to improve the accuracy and reliability of traditional liquid transfer mode of automatic clinical laboratory instruments. Methods Based on pneumatic displacement technology, the STM32 microprocessor was used as the main control chip. By real-time detection of the pressure inside the pipette, the functions of liquid level detection and abnormal sampling of suction samples were realized. Position-speed double closed-loop PID algorithm was used to achieve the precise positioning of linear stepping motor in order to improve the accuracy of sample absorption. Finally, the monitoring experiment of pipetting process and the verification experiment of pipetting accuracy were carried out to verify the liquid level detection function, the abnormal recognition function of the sample and the accuracy. Results The micropipette had higher precision, smaller error and better repeatability of liquid transfer, with the function of whole process monitoring. Conclusions The micropipette designed in this paper meets the JJG 646—2006 national design specification, and is suitable for fully automatic clinical laboratory instruments.

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