北京生物医学工程

兔眼虹膜形态随眼内压变化的体内实验研究

Experimental research on the morphological changes of rabbit irisin vivowith the rise of intraocular pressure

作者：张昆亚钱秀清吴宝平王辉刘志成

单位：首都医学大学生物医学工程学院（北京 100069）；首都医科大学临床生物力学应用基础研究北京市重点实验室（北京 100069）

关键词：虹膜；形态参数；高眼压；玻璃体灌注；青光眼

分类号：R318

出版年·卷·期（页码）：2019·38·6（605-610）

摘要：

目的获得动物急性高眼压下的虹膜形态变化，为深入认识伴虹膜形态变化的青光眼病程发展提供帮助。方法选用新西兰白兔7只，基于自主搭建的实验平台，对兔眼玻璃体进行穿刺，快速灌注生理盐水。灌注过程中，通过传感器记录前房眼压变化，通过小动物超声影像装置记录虹膜的形变图像。为了量化虹膜的形态变化，选取虹膜面积、长度、上弧长和下弧长作为虹膜的形态参数。通过图像和数据处理，获得虹膜的形态参数随眼内压的变化规律。结果玻璃体灌注过程中，眼内压升高，晶体前移，虹膜内缘前移。虹膜各形态参数随眼内压的升高均呈现下降趋势，二者关系可以通过公式

s = b₀+ b₁lnp 进行拟合，其中，s为任一形态参数，p为眼内压，b₀和b₁均为拟合参数。结论玻璃体灌注可以快速升高眼内压，并伴随虹膜的形态变化。本研究为深入了解伴虹膜形态变化的青光眼病程提供了实验基础。

Objective Our purpose is to qualify morphological changes of the iris in the acute ocular hypertension animal model, which may be helpful to know the process of angle closure glaucoma with the morphological changes of the iris. Methods Experiments are conducted on seven New Zealand white rabbits based on the self-built experimental platform. Normal saline is infused to the vitreous body. During the infusion, the sensor is used to monitor the IOP (intraocular pressure), and morphology of the iris is captured by the small animal ultrasonic imaging system. In order to quantify the morphological changes of the iris well, certain morphometric parameters are chosen such as cross-sectional area, length, upper arc length and lower arc length. Moreover, the relationships between the morphometric parameters and the IOP are obtained by image processing and data processing. Results Normal saline infusion to the vitreous body increases the IOP. With the rise of the IOP, both the lens and pupillary margin of the iris move forward. Meanwhile, the iris morphometric parameters change. Furthermore, the fitting formula between the morphological parameters and the IOP are established, which can be described as s = b₀+ b₁lnp while s is any morphometric parameter, p is IOP, b₀ and b₁ are both fitting parameters. Conclusions The vitreous body infusion could induce acute ocular hypertension with morphological changes of the iris, which gives insight to the process of glaucoma with the morphological changes of the iris.

参考文献：

[1] Huang EC, Barocas VH. Active iris mechanics and pupillary block: steady-state analysis and comparison with anatomical risk factors[J]. Annals of Biomedical Engineering, 2004, 32(9):1276-1285.

[2] Mapstone R. Mechanisms in open-angle glaucoma[J]. The British Journal of Ophthalmology, 1978, 62(5): 275–282.

[3] Wang BS, Sakata LM, Friedman DS. Quantitative parameters and association with narrow angles[J]. Ophthalmology, 2010, 117(1):11-17.

[4] Wang BS, Narayanaswamy A, Amerasinghe N, et al. Increased iris thickness and association with primary angle closure glaucoma[J]. The British Journal of Ophthalmology, 2011, 95(1): 46-50.

[5] Pant AD, Gogte P, Pathak-Ray V, et al. Increased iris stiffness in patients with a history of angle closure glaucoma: an image-based inverse modeling analysis[J]. Investigative Ophthalmology & Visual Science, 2018, 59(10): 4134-4142.

[6] Aptel F, Denis P. Optical coherence tomography quantitative analysis of iris volume changes after pharmacologic mydriasis[J]. Ophthalmology, 2010, 117(1): 3-10.

[7] Quigley HA, Silver DM, Friedman DS, et al. Iris cross-sectional area decreases with pupil dilation and its dynamic behavior is a risk factor in angle closure[J]. Journal of Glaucoma, 2009, 18(3):173-179.

[8] 张昆亚, 钱秀清, 刘志成. 基于小动物超声影像系统的高眼压下在体兔眼虹膜形态变化研究[J]. 中国医学装备, 2018, 15(11):158-162.

Zhang KY, Qian XQ, Liu ZC. Experimental research on iris profile changes under high IOP by the ultrasound system of small animals[J]. China Medical Equipment, 2018, 15(11):158-162.

[9] Zhang K, Qian X, Mei X, et al. An inverse method to determine the mechanical properties of the iris in vivo[J]. Biomedical Engineering Online, 2014, 13: 66.

[10] 王宁利, 欧阳洁, 周文炳，等. 中国人闭角型青光眼房角关闭机制多样性的研究[J]. 中华眼科杂志, 2000, 36(1): 46-51.

Wang NL, OuYang J, Zhou WB, et al. Multiple patterns of closure mechanisms in primary angle closure glaucoma in Chinese[J]. Chinese Journal of Ophthalmology, 2000, 36(1):46-51.

[11] 蒋鹏飞,李翔,彭清华.复合式小梁切除术与传统小梁切除术治疗原发性闭角型青光眼的Meta分析[J].中国医药导报, 2019,16(9): 102-105, 113.

Jiang PF, Li X, Peng QH. Meta analysis of compound trabeculectomy and traditional trabeculectomy in the treatment of primary angle-closure glaucoma[J]. China Medical Herald, 2019, 16(9): 102-105, 113.

[12] Ritch R, Chang BM, Liebmann JM. Angle closure in younger patients[J]. Ophthalmology, 2003, 110(10): 1880-1889.

[13] 李思珍, 梁远波, 王宁利. 原发性闭角型青光眼的房角关闭机制[J]. 青岛大学医学院学报, 2009, 45(1): 86-88.

[14] Levene R. A new concept of malignant glaucoma[J]. Archives of Ophthalmology, 1972, 87(5): 497-506.

[15] 张昆亚，钱秀清，刘志成.在体兔眼虹膜材料模型的确定[J].中国医学物理学杂志, 2018, 35(11): 1355-1359.

Zhang KY, Qian XQ, Liu ZC. Determination of material model for the rabbit iris in vivo[J]. Chinese Journal of Medical Physics, 2018, 35(11):1355-1359.

服务与反馈：

【文章下载】【加入收藏】

提示：您还未登录，请登录！点此登录