北京生物医学工程

胍基抗菌材料在糖尿病动物皮肤创伤中的抗菌性能研究

Antibacterial properties of guanidine materials on wounds of diabetic animal skin

作者：邱浩峰冯培培骆阳吴徐金侯文佳牟贤波竺亚斌

单位：宁波大学医学院（浙江宁波 315211）

关键词：聚六亚甲基胍；胍基聚合物; 糖尿病; 皮肤创伤; 抗菌

分类号：R318.08

出版年·卷·期（页码）：2020·39·3（233-243）

摘要：

目的糖尿病可以引起多种并发症，皮肤溃疡、溃烂是常见症状，并且经久不愈。因此，本研究拟制备一种性能优异的胍基抗菌材料，以对糖尿病患者皮肤溃疡、溃烂的治疗提供参考。方法首先将盐酸胍与己二胺发生聚合反应，合成具有良好抗菌性能的系列聚六亚甲基胍（polyhexamethylene guanidine，PHMG），并用红外光谱分析检测PHMG的分子结构；然后对其进行抗菌性能检测，菌种包括大肠杆菌、耐甲氧西林金黄色葡萄球菌MRSA USA300、铜绿假单胞菌PAO1和PGO2330；最后，构建糖尿病模型小鼠，并造成小鼠皮肤损伤，在未染菌和感染金黄色葡萄球菌（MRSA USA300）两种情况下，对PHMG的杀菌和抗感染性能进行研究。结果聚合反应发生，成功合成系列PHMG。其中，PHMG5对上述4种细菌具有很好的抗菌效果。动物实验证明，PHMG5对于糖尿病小鼠皮肤伤口具有显著抗菌性能。结论通过聚合反应可以合成系列聚六亚甲基胍，该胍基聚合物具有良好的抗菌性能，有利于糖尿病小鼠伤口的抗感染。

Objective Severe diabetic patients are always accompanied by refractory skin ulceration, since the patients possessed abnormal insulin regulation and weak immunity which leaded to the poor resistance to foreign microorganism infection. Therefore, the purpose of this study is to synthesize a guanidine-based material with excellent antibacterial properties, which will be valuable for the treatment of diabetic skin ulcers in future. Methods Polyhexamethylene guanidines (PHMGs) with good antibacterial properties were synthesized based on the polymerization reaction of guanidine diamine with hexamethylenediamine under appropriate conditions. The molecular structure of PHMG was detected by infrared spectrum analysis. Subsequently, the antibacterial properties of these materials to common bacteria were tested. The strains include: Escherichia coli(E. coli), methicillin-resistant Staphylococcus aureus (MRSA USA300), Pseudomonas aeruginosa (PAO1 and PGO2330). On this basis, diabetic mice were constructed to simulate the skin damage of clinically diabetic patients. The bactericidal and anti-infection properties of PHMGs were investigated via S. aureus MRSA USA300 diabetic wound infection model in mice. Results Infrared spectroscopy confirmed the occurrence of the polymerization reaction. The minimum bacteriostatic concentration and bacteriostatic zone experiments showed that PHMGs had good bacteriostatic effects on the above four kinds bacteria. The in vivo experiment verified that PHMGs had significant inhibitory effects on skin wounds of diabetic mice. Conclusions A series of polyhexamethylene guanidine were synthesized through polymerization between guanidine diamine and hexamethylenediamine. The guanidine-based polymer has good bacteriostatic properties and is beneficial to the anti-infection of wounds in diabetic mice.

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