Objective Atrial fibrillation (af) is one of the most common arrhythmias in clinic. Left atrial appendage occlusion is a kind of new technology that is different from the development of traditional medicine with minimally invasive interventional auricle to prevent thromboembolism. LAA occluder?in the body under a cycle load could lead to a fatigue fracture, which is life-threatening, and the rupture of the barrier film caused by the blood flow on the barrier film will lead to the poor sealing effect. Therefore, a kind of fatigue durability test to evaluate?the fatigue resistance of occluder?in human bodies needs to be established.  Methods The research was conducted by first choosing the diameter control method for the fatigue test according to the pharmaceutical industry standard YY 0808-2010 and then determining the specification and amplitude of left atrial appendage in the fatigue test. Finally, the state of the occluder after 19 million fatigue (endothelialization time point) and 380 million fatigue (10 years fatigue time point) were obtained in accordance with the test steps.  Results After the fatigue test of 19 million times, there was no damage to membrane, loosing of the suture line, breakage of the metal parts, or moving fatigue of the sealer. After the fatigue test of 380 million times, the metal parts of the left atrial appendage were free from fracture and wear. Conclusion A convenient and efficient method of fatigue durability evaluation can be provided for enterprises and scientific research institutions that study the left atrial appendage on the basis of the research.