北京生物医学工程

水性组织透明化技术的研究进展与应用

Research progress and application of hydrophilic reagent-based tissue clearing technology

作者：吴昊妍詹琰静张士文

单位：口腔疾病研究国家重点实验室，国家口腔疾病临床医学研究中心，四川大学华西口腔医学院（成都 610041）口腔疾病研究国家重点实验室，国家口腔疾病临床医学研究中心，四川大学华西口腔医院种植科（成都 610041）

关键词：组织透明化技术；水性透明化；光散射；临床应用；三维成像

分类号：R318

出版年·卷·期（页码）：2021·40·3（318-323）

摘要：

由于生物样本的不透明性，以及组织深入成像的清晰度和深度不佳，因此大型、厚组织3D成像技术的发展与创新成为近年来的研究热点。组织透明化机技术是通过各种物理或化学的方式将组织变透明，并结合荧光显微镜来实现组织或器官三维成像的新技术，因其良好的荧光保存及透明效果，在软组织领域，尤其是神经领域得到了越来越广泛的使用。近年来，各种新型的组织透明化技术不断被报道出来，本文就被动浸润法水性透明化技术、水化法水性透明化技术、水凝胶嵌入法水性透明化技术的研究进展及应用做一综述。

The development and innovation of 3D imaging technology for large and thick tissues have become a research hotspot in recent years due to the opacity of biological specimens and the lack of clarity and depth of tissue sample imaging. The hydrophilic reagent-based tissue clearing technology, through a variety of physical or chemical methods to clear tissues, enables the three-dimensional imaging of tissues or organs with fluorescence microscopes, which has been widely adopted to clear soft tissues, especially nerves because of its satisfactory fluorescence preservation and clearing effects. An increasing number of new tissue clearing techniques have constantly been reported in the last few years. In this paper, the research progress and application of passive infiltration water - based transparency technology, hydration water transparency technology and hydrogel embedding technique for waterborne transparency will be reviewed.

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