北京生物医学工程

基于原子力显微镜压痕技术的大鼠小梁网组织弹性模量测定

Determination of elastic modulus of rat trabecular meshwork by atomic force microscope indentation technique

作者：王川李林刘志成

单位：1首都医科大学燕京医学院（北京101300） 2首都医科大学生物医学工程学院（北京 100069） 3临床生物力学应用基础研究北京市重点实验室（北京 100069） 通信作者：刘志成，教授，博士研究生导师。E-mail：zcliu@ccmu.edu.cn

关键词：小梁网组织；原子力显微镜；弹性模量，压痕技术；Hertz模型

分类号：R318.01

出版年·卷·期（页码）：2021·40·4（354-359）

摘要：

目的探索基于原子力显微镜压痕技术确定大鼠小梁网组织弹性模量的方法，为揭示小梁网组织力学特性与小梁网通道房水外流阻力之间的关系等研究奠定基础。方法首先利用大鼠眼球组织切片获取大鼠小梁网组织与毗邻组织之间的距离信息和结构信息；其次利用AFM压痕实验获取角膜-角巩膜缘-小梁网-巩膜方向的弹性模量与相对位置的关系；综合对比以确定小梁网组织测试区域。结果探索了一种基于原子力显微镜压痕实验定位小梁网组织并获取该组织弹性模量的方法，利用该方法获取了大鼠眼球鼻侧、颞侧、上侧、下侧4个位置处的小梁网组织弹性模量范围为300～600 Pa。结论本研究给出了一种基于AFM压痕技术确定大鼠小梁网组织弹性模量的方法，该方法提供了AFM测试环境下小梁网组织区域的位置参考，优势在于可快速定位到大鼠小梁网组织区域。

Objective To explore the method of determining the elastic modulus of rat trabecular meshwork（TM） based on atomic force microscope (AFM) indentation technique, and lay the foundation for revealing the relationship between the mechanical properties of TM and the outflow resistance. Methods Firstly, obtain the distance and structure information of TM and adjacent tissues by using the rat eye tissue section; secondly, the relationship between elastic modulus and relative position of cornea-corneoscleral limbus-TM-sclera was obtained by AFM indentation; the test area of TM was determined by comprehensive comparison. Results Explored a method for locating TM and obtaining its elastic modulus based on AFM indentation experiment. The elastic modulus of TM at nasal, temporal, superior and inferior sides of the eyeball was obtained by this method, which ranged from 300Pa to 600Pa. Conclusions This study provides a method to determine the elastic modulus of rat TM based on AFM indentation. This method can provide the location reference of TM area in AFM test environment, and the advantage is that it can quickly locate the TM tissue area in rats.

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