北京生物医学工程

碳纤维/磁性纳米颗粒/高分子共轴纤维复合支架构建及其对成纤维细胞的作用研究

Fabrication of carbon fiber/magnetic nanoparticles/ coaxial polymerfiber composite scaffold and the effect on fibroblasts

作者：刘青桥郝博雅刘文昊童元建周航刘健许海燕

单位：中国医学科学院基础医学研究所，北京协和医学院基础学院（北京100005） 中国医学科学院北京协和医院（北京100730） 北京化工大学碳纤维及功能高分子教育部重点实验室（北京 100029） 通信作者:许海燕,E-mail: xuhy@ pumc.edu.cn;刘健,E-mail ： liujian@ ibms. pumc. edu. cn

关键词：静电纺丝;复合支架;碳纤维;磁性纳米颗粒;成纤维细胞

分类号：R318. 08

出版年·卷·期（页码）：2022·41·1（1-7）

摘要：

目的构建碳纤维/磁性纳米颗粒/高分子共轴纤维复合支架，研究支架的微观形貌及理化性质,初步评价支架对成纤维细胞生长的作用。方法采用共轴静电纺丝技术制备纤维薄膜，芯层为聚乳酸，壳层为明胶，各层均含有Fe3O4纳米颗粒，在薄膜层中间加入碳纤维，得到复合支架。通过扫描电子显微镜观察电纺丝纤维及碳纤维的微观结构，使用四探针测试仪、震荡样品磁强计、电子万能试验机和差示扫描量热仪检测支架的电导率、磁滞回线、弹性模量和热行为。用激光共聚焦显微镜观察成纤维细胞形貌,利用细胞增殖-毒性检测试剂盒评价细胞活性。结果电纺丝纤维呈无规缠结状态，碳纤维均匀散布在电纺丝薄膜上。Fe3O4纳米颗粒和碳纤维的加入均提高了复合纤维薄膜的电传导性和弹性模量,Fe3O4纳米颗粒还赋予复合薄膜以超顺磁响应性。细胞在支架上能正常生长,与对照组相比,复合支架上细胞的骨架蛋白F-actin表达水平显著升高，且骨架纤维更具张力。结论复合支架获得了超顺磁响应性和导电性,并可显著促进成纤维细胞的生长。

Objective To fabricate carbon fiber/magnetic nanoparticles/coaxial polymer fiber composite scaffolds and investigate the morphology and physicochemical properties of the scaffolds, and evaluate the effect of scaffolds on the growth of fibroblasts. Methods The fiber films were fabricated by coaxial electrospinning with gelatin ( Gel) as shell layer and polylactic acid ( PLA) as core layer； meanwhile, Fe304 magnetic nanoparticles were added into the gelatin and PLA. Carbon fiber was placed in the middle of the filmsto obtain the composite scaffolds. The microstructure of electrospun fiber and carbon fiber was observed by scanning electron microscope (SEM). The conductivity,hysteresis loop,elastic modulus and thermal behavior of the scaffolds were measured by four-point probes, vibrating sample magnetometer, universal material testing machine and differential scanning calorimeter, respectively. The growth of mouse embryonic fibroblast cell lines ( NIH - 3T3 ) on the composite scaffolds was observed by confocal microscope,and cell viability was profiled by cell counting kit-8 (CCK-8) assay. Results The electrospun fibers were random tangled, and the carbon fibers were evenly distributed on the electrospun films. The addition of Fe3O4 magnetic nanoparticles and carbon fibers improved the electrical conductivity and elastic modulus of the composite fiber film,and Fe3O4 nanoparticles also endowed the composite films with superparamagnetism. Fibroblasts could grow and proliferate normally on different scaffolds. Compared with fibroblasts grown on PLA/Gel, the expression level of F-actin in fibroblasts grown on mag-PLA/Gel/CF was significantly increased, and the cytoskeleton was more tensive. Conclusions The composite scaffolds had both superparamagnetism and conductivity,which can significantly promote the growth of fibroblasts.

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