北京生物医学工程

生物瓣抗钙化检测方法的研究

Study on anti-calcification detection method of biological valve

作者：于秋航段苏然杨柳姜爱莉付海洋王召旭

单位：烟台大学（山东烟台264005 中国食品药品检定研究院（北京102629）济宁市食品药品检验检测中心（山东济宁 272100） 通讯作者：1.付海洋。E-mail:fuhaiyangnifdc@163.com    2.姜爱莉。E-mail:jal9035@163.com

关键词：生物材料；钙；火焰-原子吸收分光光度法；电感耦合等离子体发射光谱法

分类号：R318.04

出版年·卷·期（页码）：2022·41·1（49-53）

摘要：

目的对火焰原子吸收分光光度法（flame atomic absorption spectrophotometry, FAAS）与电感耦合等离子体原子发射光谱法（inductively coupled plasma atomic emission spectrometry，ICP-OES）进行方法学验证研究，以探究两种方法应用于检测生物材料钙含量的可行性，为制定生物瓣抗钙化性能检测方法标准提供参考。方法将生物瓣植入3周刚断乳雄性Wistar大鼠背部皮下，8周后取出植入物，用FAAS法和ICP-OES法检测植入物中钙含量，并研究两种方法的线性、专属性、重复性、精密度、准确度及样品检测结果的差异。结果当生物瓣的钙质量浓度为0~5.0 μg/mL时两种方法的线性关系均良好，相关系数大于0.999。ICP-OES法测定时定量限更低、测准确性更好，与FAAS法相比更为优秀，且两种方法的回收率与相对标准偏差都能满足对生物材料中钙含量测定的要求。结论 FAAS法与ICP-OES法均可作为标准方法用于生物瓣中钙含量的测定，FAAS需要加入掩蔽剂，但测定过程灵活，可通过预估将检验液中钙浓度稀释至标曲浓度范围内后测定；ICP-OES受干扰少，但不能预估检验液的钙含量，因此适用于钙含量范围一定的大量样品的检测工作。

Objective The two methods of flame atomic absorption spectrophotometry （FAAS） and inductively coupled plasma atomic emission spectrometry （ICP-OES）are verified and compared to explore the feasibility of applying the two methods to detect the calcium content of biomaterials, so as to provide reference for formulating the detection method standard of anti calcification performance of biological valve. Methods The biological valve is implanted subcutaneously into the back of three week weaned male Wistar rats, after eight weeks, the implants are removed, the calcium content in the implants is detected by FAAS and ICP-OES, and the linearity, specificity, repeatability, precision, accuracy and sample detection results of the two methods are compared. Results When the calcium concentration of biological valve is 0 ~ 5.0 μg / mL, the linear relationship between the two methods is good, and the correlation coefficient is greater than 0.999. Compared with FAAS method, ICP-OES Method has lower quantitative limit and better accuracy, and the recoveries and relative standard deviations of the two methods can meet the requirements for the determination of calcium content in biomaterials. Conclusions Both FAAS and ICP-OES can be used as standard methods for the determination of calcium in biological valve. FAAS needs to add masking agent, but the determination process is flexible. The calcium concentration in the solution to be measured can be diluted to the standard curve concentration by estimation; ICP-OES is less disturbed, but it can not predict the calcium content of the test solution, so it is suitable for the detection of a large number of samples with a certain range of calcium content.

参考文献：

[1] Li RL, Luo CW, Ho YC, et al. Heart valve operations associated with reduced risk of death from mitral valve disease but other operations associated with increased risk of death: a national population-based case-control study [J] Journal of Cardiothoracic Surgery,2019, 14(1):165.

[2] 何盛南, 曲龙, 邹志成等. 生物心脏瓣膜移植研究进展[J]. 器官移植, 2017, 8(5): 410- 412.

[3] Schoen FJ, Levy RJ. Calcification of tissue heart valve substitutes: progress toward understanding and prevention [J]. Annals of Thoracic Surgery,?2005, 79(3):1072-1080.

[4] 杨学永, 胡素平, 刘兵等. 生物心脏瓣膜钙化及预防[J].武警医学,2004,15(8):629-630.

[5] 付海洋,于秋航,段苏然,等.人工生物心脏瓣膜钙化机制及评价研究进展[J].中国医刊,2021,56(8): 840-843.

Fu HY, Yu QH, Duan SR, et al. Research progress on calcification mechanism and evaluation of artificial biological heart valve [J]. Chinese Journal of medicine, 2021, 56 (8): 840-843.

[6] 张丹丹, 付海洋, 刘丽萍,等. 应用大鼠皮下植入模型评价人工生物心脏瓣膜钙化[J]. 中华临床医师杂志(电子版), 2015, 9 (21): 3909- 3917.

Zhang DD, Fu HY, Liu LP, et al. Evaluating the calcification of bioprosthetic heart valve substitute by the rat subcutaneous implantation model [J] . Chinese Journal of clinicians (Electronic Edition), 2015, 9(21): 3909 – 3917.

[7] 马玉婷,李正,董丹丹,等.火焰原子吸收光谱法快速测定保健品中的钙[J].食品工业,2018,39(9): 299-301.

Ma YT, Li Z, Dong DD, et al. Rapid determination of calcium in health products by flame atomic absorption spectrometry [J]. Food industry, 2018, 39 (9): 299-301.

[8] 陈华林,冯忠琼,张枝健,等.EDTA滴定法测定钙离子浓度的影响因素与优化[J].西南民族大学学报(自然科学版),2020,46(6): 578- 585.

Chen HL, Feng ZQ, Zhang ZJ, et al. Influence factors and optimization on the EDTA titration of calcium [J]. Journal of Southwest University for Nationalities (Natural Science Edition), 2020, 46 (6): 578-585.

[9] 张歆皓,邓阿利,王会东,等.儿童补钙剂中钙含量的测定研究[J].赤峰学院学报(自然科学版),2016,32(8):7- 10.

[10] 张楠,孙开奇,沙博郁,等.湿法消解-电感耦合等离子体发射光谱法测定保健食品中7种常见矿物质元素[J].食品安全质量检测学报,2021,12(2): 800- 804.

Zhang N, Sun KQ, Sha BY, et al. Determination of seven common mineral elements in health food by wet digestion inductively coupled plasma atomic emission spectrometry [J]. Journal of Food Safety and Quality Inspection, 2021,12 (2): 800-804.

[11] 李波平,陈文锐,卢丽等.保健品中钙含量测定的ICP-OES法和FAAS法比较[J].食品工业,2020,41(6):307- 310.

Li BP, Chen WR, Lu L, et al. Comparison the detection methods of ICP-OES and FAAS on calcium element in health care products [J]. Food industry, 2020, 41 (6): 307-310.

[12] 国家药典委员会.中华人民共和国药典四部[M].北京:中国医药科技出版社,2020:480-483

[13] 林丽钦.原子吸收测定酸雨钾,钠,钙,镁干扰因素探讨及其消除[J].福建分析测试,2007,16(4):25- 29.

Lin LQ. Study on interferences in simultaneous determination of K、Na、Ca、Mg in Acidrain by Atomic Absorption Spectrometry [J] . Fujian Analysis and Testing, 2007,16 (4): 25-29.

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