北京生物医学工程

伊班膦酸钠联合骨髓间充质干细胞干预治疗骨质疏松动物的骨组织生物力学性能研究

Study on the biomechanical properties of bone tissue of osteoporosis animals treated with ibandronate combined with bone marrow mesenchymal stem cells

作者：刘晓辉秦璇杜静郝明高志达潘铄胡亚宁田智广

单位：石家庄市人民医院骨一科（石家庄市 050000） <p>通讯作者：刘晓辉。E-mail:ortholxh@163.com</p> <p> </p>

关键词：骨质疏松；动物模型；骨髓间充质细胞；骨密度；生物力学

分类号：R318.01

出版年·卷·期（页码）：2022·41·3（280-285）

摘要：

目的验证骨髓间充质干细胞(bone mesenchymal stem cells ，BMSCs)联合伊班膦酸钠干预治疗骨质疏松动物后骨的压缩、弯曲等力学性能恢复的效果。方法取5月龄雌性SD大鼠60只，随机分为正常对照组12只，骨质疏松动物模型组12只，骨质疏松动物模型以伊班膦酸钠干预组12只，骨质疏松动物模型以骨髓间充质干细胞干预组12只，骨质疏松动物模型以伊班膦酸钠联合骨髓间充质干细胞干预组12只。以摘除大鼠卵巢的方法建立骨质疏松动物模型。大鼠饲养3周后，分别以伊班膦酸钠、骨髓间充质干细胞、伊班膦酸钠联合骨髓间充质干细胞对骨质疏松动物进行干预治疗，于给药7周后检测各组大鼠血清骨特异性碱性磷酸酶( bone alkaline phosphotase，BALP)、抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase-5b ，TRACP-5b)，并取大鼠股骨进行压缩、弯曲力学性能实验，检测胫骨骨密度(bone mineral density，BMD)。结果以伊班膦酸钠联合骨髓间充质干细胞干预组胫骨 BMD、血清BALP高于模型组、以伊班膦酸钠干预组、以骨髓间充质干细胞干预组，其血清TRACP -5b 小于骨质疏松动物模型组、以伊班膦酸钠干预组、以骨髓间充质干细胞干预组；股骨各项压缩和弯曲力学性能大于模型组、以伊班膦酸钠干预组、以骨髓间充质干细胞干预组，差异具有统计学意义（P<0.05）。结论通过以伊班膦酸钠联合骨髓间充质干细胞干预治疗可使骨质疏松动物胫骨BMD、血清ALP升高、TRACP-5b水平下降，股骨的压缩、弯曲强度和韧性可以得到一定恢复。

Objective This paper verifies the effect of bone marrow mesenchymal stem cells (BMSCs) combined with ibandronate intervention on the recovery of mechanical properties such as compression and bending of bone after treatment of osteoporotic animals.Methods Sixty 5-month-old female SD rats were randomly divided into normal control group (n = 12), osteoporosis animal model group (n = 12), osteoporosis animal model with ibandronate intervention group (n = 12), osteoporosis animal model with BMSCs intervention group (n = 12), osteoporosis animal model with ibandronate combined with BMSCs intervention group (n = 12). The animal model of osteoporosis was established by ovariectomy. After 3 weeks of feeding, the rats were intervened with ibandronate sodium, BMSCs, ibandronate sodium combined with BMSCs. After 7 weeks of administration, the serum levels of bone alkaline phosphatase (BAL), tartrate-resistant acid phosphatase 5b (TRACP-5b) were detected, the femurs of rats were taken for compression and bending tests, and the bone mineral density of tibia (BMD) was also measured. Results The BMD of tibia and serum BALP in ibandronate sodium combined with BMSCs intervention group were higher than those in model group, ibandronate sodium intervention group and BMSCs intervention group,while the level of serum TRACP-5b in ibandronate combined with BMSCs intervention group was lower than that in osteoporosis animal model group, ibandronate group and BMSCs intervention group; Compression and bending mechanical properties of femur in ibandronate sodium combined with BMSCs intervention group were greater than those in model group, ibandronate sodium intervention group and BMSCs intervention group,the difference was significant (P < 0.05).

Conclusion: Osteoporosis animals can recover with higher level of the BMD and serum BALP, lower level of TRACP-5b by using bisphosphonate combined with BMSCs stem cells, the femoral compression and bending strength and toughness were also greater.

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