北京生物医学工程

氧化铁纳米颗粒对静止状态白血病细胞的作用研究

The effects of iron oxide nanoparticles on quiescent leukemia cells

作者：李宛清张雪温涛刘健孟洁许海燕

单位：中国医学科学院基础医学研究所，北京协和医学院基础学院（北京  100005）<br />通信作者：许海燕，E-mail：xuhy@pumc.edu.cn；孟洁，E-mail：mengjie@ibms.pumc.edu.cn

分类号： R318.04

出版年·卷·期（页码）：2023·42·1（52-56）

摘要：

目的研究二巯基丁二酸修饰的四氧化三铁纳米颗粒（DMSA-Fe3O4 NPs）对静止状态白血病细胞的作用及机制。方法利用透射电镜观察纳米颗粒形貌；用低血清培养基（0.5% FBS）诱导细胞进入静止状态，采用普鲁士蓝染色法与邻二氮菲铁定量法检测DMSA-Fe3O4 NPs进入Kasumi-1细胞的情况；通过乳酸脱氢酶法、细胞氧化应激荧光探针和凋亡检测试剂分析 DMSA-Fe3O4 NPs对Kasumi-1细胞的作用。结果 DMSA-Fe3O4 NPs可进入静止状态的Kasumi-1细胞，细胞内铁含量与DMSA-Fe3O4 NPs浓度成正比；与此同时，细胞活性降低，细胞内ROS与脂质过氧化物水平升高，细胞发生凋亡。正常培养的Kasumi-1细胞活性和氧化应激水平不受DMSA-Fe3O4 NPs的影响。结论 DMSA-Fe3O4 NPs可进入静止状态的白血病细胞并引起细胞凋亡。

Objective To investigate the effect of dimercaptosuccinic acid modified ferric oxide nanoparticles (DMSA-Fe3O4 NPs) on quiescent acute myeloid leukemia cell line (Kasumi-1). Methods The morphology of DMSA-Fe3O4 NPs was observed by transmission electron microscopy. The low serum containing medium (0.5% FBS) was applied to induce Kasumi-1 cells to the quiescent state. The cellular uptake of DMSA-Fe3O4 NPs was analyzed by Prussian blue staining and phenanthroline spectrophotometric analysis. The release of lactate dehydrogenase, reactive oxidative species and apoptosis of Kasumi-1 treated with DMSA-Fe3O4 NPs at different concentrations were examined. Results The DMSA-Fe3O4 NPs entered the quiescent Kasumi-1 cells and the content of intracellular iron increased in a nanoparticles dose dependent manner. The viability of the quiescent Kasumi-1 cells was significant decreased and the intracellular ROS and lipid peroxide were increased as well. The Kasumi-1 cells cultured with the complete culture medium (10% FBS) was not affected by DMSA-Fe3O4 NPs. Conclusions DMSA-Fe3O4 NPs could preferably enter the quiescent Kasumi-1 cells, which resulted in the upregulation of intracellular oxidative stress and cell apoptosis.

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