北京生物医学工程

心肌细胞电脉冲作用下电场分布仿真结果与分析

Simulation results and analysis of electric field distribution under the action of electrical pulses in cardiac muscle cells

作者：朱宇成王群山莫斌峰牛金海

单位：上海交通大学生物医学工程学院(上海  200240)<br />上海交通大学医学院附属新华医院(上海  200092)<br />通信作者：牛金海，E-mail：jhniu@sjtu.edu.cn；莫斌峰，E-mail：mobinfeng@xinhuamed.com.cn 

关键词：心脏消融；心肌细胞；脉冲电场；电势分布；仿真分析

分类号：R318.04

出版年·卷·期（页码）：2023·42·1（67-73）

摘要：

目的细胞电脉冲刺激仿真是研究心脏电脉冲消融的一种仿真方式，本文建立椭球形细胞电脉冲刺激仿真模型，模拟心肌细胞受到电脉冲刺激下的情况，研究电场入射方向和细胞长度对其电场分布和跨膜电位的影响。方法通过COMSOL5.5软件进行仿真，以球形细胞模型为基础，在边长60μm的立方体空间中建立椭球形细胞模型。于垂直于Z轴的两面施加2.4 V电压，用以模拟心肌细胞在外加匀强电场作用下的电势分布情况。改变脉冲电场与细胞长轴的夹角，研究0、30°、60°、90°的不同电场入射角度对心肌细胞电势分布和跨膜电位的影响。保持入射角为0，研究跨膜电位最大值与细胞长轴直径的关系。结果对于椭球形的心肌细胞，电场的入射角从0°增大到90°时，跨膜电位从1.068V减小至0.373V，同时最大跨膜电位的位置也发生改变。入射角为0°时，跨膜电位最大值V与细胞长轴直径D的线性回归方程为V_2=81.1916+38.6079D_2 ，r^2=0.9981。结论电场入射角越大，细胞跨膜电位越低；细胞长轴直径越长，跨膜电位最大值越大。该研究对后续心肌细胞电脉冲刺激实验及心脏电脉冲消融的临床试验具有参考意义。

Objective Cell electrical pulse stimulation simulation is a method to study cardiac electrical pulse ablation. In this paper, an ellipsoidal cell electrical pulse stimulation simulation model is established to simulate the situation of myocardial cells stimulated by electrical pulses, and to study the electric field distribution direction and cell length on the electric field and transmembrane potential. Methods Using COMSOL5.5 software for simulation, based on the spherical cell model, an ellipsoidal cell model is established to simulate the potential distribution of cardiomyocytes under the action of a uniform electric field. Apply 2.4 V voltage on both sides perpendicular to the Z axis to simulate the potential distribution of myocardial cells under the action of an external uniform electric field. By changing the angle between the pulsed electric field and the long axis of the cell, the effects of different electric field incidence angles of 0 °, 30 °, 60 ° and 90 ° on the potential distribution and transmembrane potential of the myocardial cell are studied. The relationship between the maximum transmembrane potential and the diameter of the long axis of cells is studied by keeping the angle of incidence at 0.Results For ellipsoidal cardiomyocytes, when the incidence angle of the electric field increases from 0 ° to 90 °, the transmembrane potential decreases from 1.068V to 0.373V, and the position of the maximum transmembrane potential also changes. When the incidence angle of the electric field is 0 °, the linear regression equation between the maximum transmembrane potential V and the cell long axis diameter D is V_2=81.1916+38.6079D_2 ，r^2=0.9981. Conclusions The greater the incident angle of electric field, the lower the transmembrane potential of cells; The longer the cell long axis diameter is, the greater the transmembrane potential is. This study has reference significance for the subsequent experiment of myocardial cell electric pulse stimulation and the clinical trial of cardiac electric pulse ablation.

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