北京生物医学工程

唾液的拉曼光谱用于疾病诊断的初步研究

Preliminary Study on Disease Diagnosis by Surface Enhanced Raman Spectroscopy with Human Saliva Sample

作者：姚雨露崔子健刘春伟焦义郑文新王燕曲典汪泓陈安宇

单位：首都医科大学生物医学工程学院（北京100069）

分类号：

出版年·卷·期（页码）：2011·30·2（137-140）

摘要：

表面增强拉曼光谱技术是近年来快速发展的一种痕量特征标记性物质检测技术，可达到分子识别水平，且具有快速、测量条件要求低、可重复性好、无污染等优点；人体唾液中含有大量体征相关的特异性蛋白质和分泌物质，唾液检测具有无创、获取方便、适合开展大规模普查等优点。表面增强拉曼光谱检测技术可以快速对唾液进行检测，二者的结合有可能成为一种适合普查的快速检测方法。本文采用便携式表面增强拉曼光谱检测仪器对44例吸毒者、45例艾滋病患者、11例肺癌患者和11例乳腺癌患者以及55例健康对照组的唾液进行测试，获取所有样本的拉曼光谱。应用支持向量机和随机森林方法对以上样本的拉曼光谱数据进行统计学分类。分析结果证实，对唾液样本的拉曼光谱检测技术可以有效地区分上述患者与健康人群。采用表面增强拉曼光谱进行唾液检测可能成为某种疾病早期诊断的一种有效方法。

Surface enhanced Raman spectroscopy (SERS) is a trace amount substance detecting technique developing quickly in recent years. It is a physical method at molecular levels, with advantages such as quick examination, low requirements on detecting conditions, good reproducibility, and no contamination. Human saliva contains abundant proteins and substances which are related to human body’s signs, and many diseases can be diagnosed by analyzing saliva features. Additionally saliva samples can be collected conveniently and non-invasively which examination is appropriate for large-scale health surveys. SERS can detect saliva samples quickly with wide prospects. In this paper, SERS was employed to test the saliva samples from 44 drug addicts, 45 AIDS patients, 11 lung cancer patients, 11 breast cancer patients and 55 healthy controls. All spectra values of saliva samples were obtained and analyzed by methods of support vector machine and random forests respectively. The results indicated that the spectra of patients could be separated from healthy controls by statistical analysis, and it supposed the saliva test using SERS could be an effective approach for early diagnosis of certain diseases.

参考文献：

［1］Fitzmaurice M, Haka AA, Volynskaya Z, et al. Raman spectroscopy: development of clinincal applications for breast cancer diagnosis［J］. SPIE, 2005, 1: 5862.
［2］Paul Denny, Fred K Hagen, Markus Hardt. The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions［J］. Proteome Res, 2008, 7 (5): 1994-2006.
［3］英国科学家称检测唾液就可诊断早期癌症［EB/OL］.［2010-12-20］.http://tech.sina. com.cn/ology/2000-08-02/32396.shtml.
［4］Tabak LA．A revolution in biomedical assessment：the developmentof salivary diagnostics［J］. Dent Edue, 2001, 65: 1335-1339.
［5］郭丽宏,俞光岩,施文元.唾液检测的研究进展［J］.现代口腔医学杂志, 2009, 23 (l): 1-3.
［6］Moskovits M. Surface-enhanced Raman spectroscopy［J］. Rev Mod Phys, 1985, 57 (3): 783-826.
［7］Otto A, Mrozek I, Grabhorn H, et al. Surface-enhanced Raman scatting［J］. Phys: Condensed Matter, 1992, 4: 1143-1212.
［8］陈安宇，王燕，焦义，等. 基于表面增强拉曼检测技术的唾液检测研究［J］. 中国医疗设备，2008，23(11)：4-6.
［9］周增会，王桂英，徐至展.表面增强拉曼光谱术在生命科学及单分子研究中的应用与进展.激光与光电子学进展［J］，2005, 42 (10): 9-13.
［10］Koglin E, Sequaris JM. Topics in Current Chemistry［M］. Berlin: Springer-Verlag, 1986: 134.
［11］赵金涛,陈大鹏,张鹏翔,等.“冰毒”拉曼散射振动模式的研究［J］.光谱学与光谱分析，1999, 19 (5): 687-690.
［12］曲典,王燕,焦义，等.基于表面增强拉曼技术的唾液筛查“冰毒”依赖者方法初探［J］.中国卫生检验杂志, 2009, 19 (11): 2558-2559.
［13］Tatiana A, Anatoly GS, Vladimir GY, et al. Drying drops of biological liquids: dynamics of the optical and mechanical properties［J］. Application in rapid medical diagnostics, SPIE, 2005, 188: 5692.
［14］Kiang Wei Kho, Olivo Malini, Ze Xiang Shen, et al. Surface enhanced Raman spectroscopic (SERS) study of saliva in the early detection of oral cancer. SPIE, 2005, 5702: 88-91.
［15］刘敬华，华琳,王燕，等. 表面增强拉曼光谱无创检测艾滋病的方法初探.北京生物医学工程杂志, 2009, 28 (5): 528-530.

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