北京生物医学工程

银纳米颗粒对神经元的毒性研究

Toxicity of Silver Nanoparticles to the Neurons

作者：周国凤汤京龙熊玲周亮许建霞邵安良杨丽娜陈艳梅李雪飞奚廷斐

单位：温州医学院（浙江温州325035）

关键词：银纳米颗粒；神经元；毒性

分类号：

出版年·卷·期（页码）：2011·30·3（301-305）

摘要：

目的研究不同浓度的银纳米颗粒对神经元毒性的剂量-效应关系，探索银纳米颗粒对神经元的毒性机理。方法首先培养一种活性好、生长状态优良的原代神经元，将不同浓度（2.5~500μg/mL）的银纳米颗粒加入神经元中作用24h后，通过MTT法计算细胞增殖率，并分析不同浓度的银纳米颗粒对神经元毒性的剂量-效应关系。结果在25~250μg/mL范围内，细胞数量与形态呈现不同的变化，银纳米颗粒浓度与细胞增殖率呈负相关。结论在25~250μg/mL的浓度范围内，银纳米颗粒对神经元细胞的毒性呈剂量-效应关系。

Objective To study the dose-effect relationship between silver nanoparticles with different concentrations and the neurotoxicity, and also explore the mechanism of silver nanoparticles to neurotoxicity. Method A group of primary neurons in excellent growth status was cultured, and then contacted with different concentrations (2.5-500μg/ml )of silver nanoparticles for 24h. After that we calculated the cell proliferation rate by MTT test and analyzed the dose-effect relationship between the concentrations of silver nanoparticle with neurotoxicity. Result Within the silver nanoparticle concenrtation of 25-250μg/ml, both cellular mount and morphology changed, and it was showed anticorrelation between silver nanoparticle concentration and cell proliferation rate. Conclusion Within the sliver nanoparticles concenrtation of 25-250μg/ml, silver nanoparticles shows a dose-effect relationship on neurotoxicity.

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