Objective To evaluate the impact of quartz crystal preparation processes on the piezoelectric immunosensor. Methods Mixtures of nanogold sol(NGS)and multi-walled carbon nanotubes(MWCNTs)were used as the carrier of immobilization for Treponema pallidum(TP)genetic engineering antigen，which was immobilized on the four kinds of quartz crystals：nickel-plated gold electrodes，nickel-free gold electrodes，nickel-plated silver electrodes，nickel-free silver electrodes respectively. Anti-TP positive serum and negative serum were detected eight times respectively. The impact of quartz crystal preparation processes on the piezoelectric immunosensor chip was evaluated with the indexes as zero drift，water droplets on surface adhesion，the immobilization amount of antigens，settling time，the amount of antigen shedding，response value and coefficient of variation(CV). Results The comprehensive evaluation of nickel-plated gold electrodes to the preparation of self-assembly piezoelectric immunosensor chip for detecting TP is the best. Conclusions Nickel-plated or nickel-free and the selection of metal electrode are two important factors for the preparation of piezoelectric immunosensor chip.