北京生物医学工程

Tween_20_对联吡啶钌电化学发光的增敏作用

Sensitization of Tween 20 on Ru(bpy)2+3 electrochemiluminescence

作者：李志英王捷许喜林陈健刘仲明

单位：广州军区广州总医院医学实验科(广州510010)

关键词： Ru(bpy)2+3；电化学发光；Tween 20;增敏效应

分类号：

出版年·卷·期（页码）：2012·31·4（402-406）

摘要：

目的为进一步提高联吡啶钌电化学发光检测的灵敏度，研究了非离子表面活性剂Tween 20对联吡啶钌电化学发光的增敏作用。方法对Tween 20的浓度进行优化后，在最佳添加浓度0.1%的条件下，测试联吡啶钌的灵敏度、线性范围和稳定性。最后，探讨了Tween 20对三丙胺电化学行为的影响。结果 Tween 20浓度为0.1%时，对联吡啶钌电化学发光的增敏作用最佳。在此浓度下，联吡啶钌的检测灵敏度提高了100倍，在浓度为5×10-11～1×10-7mol/L范围内与电化学发光强度呈良好的线性关系，且Tween 20能促进三丙胺在电极表面的直接氧化并增强其氧化电流。结论 Tween 20对联吡啶钌的电化学发光具有较强的增敏作用。

Objective To improve the sensitivity of the Ru(bpy)2+3 electrochemiluminescence (ECL) detection, the sensitization of nonionic surfactant Tween 20 on the ECL of Ru (bpy)2+3 was studied. Methods The concentration of Tween 20 added was optimized firstly. In the optimum concentration of Tween 20 as 0.1%, the sensitivity, linear range and repeatability of Ru(bpy)2+3 were tested. Then, the effects of Tween 20 on the electrochemical behavior of tripropylamine were discussed.Results When the concentration of Tween 20 was 0.1%, the sensitization on the ECL was best. In this concentration, the sensitivity of Ru(bpy)2+3 increased 100 times, and there was a good linear relationship between the concentration of Ru(bpy)2+3 and ECL intensity in the range of 5×10-11 to 1×10-7mol/L. Moreover, Tween 20 could promote the direct oxidation of tripropylamine on the electrode surface and enhance the oxidation current. Conclusions Tween 20 has strong sensitization of on the ECL of Ru(bpy)2+3.

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