北京生物医学工程

松质骨移植材料孔隙率的显微CT和组织切片测试方法比较

The cancellous bone graft substitute porosity tested by the micro CT image method and the biopsy image method

作者：曹帅赵改平颜文涛

单位：上海理工大学生物力学与康复工程研究所(上海200093)

分类号：

出版年·卷·期（页码）：2012·31·5（478-481）

摘要：

目的采用显微CT法和组织切片法对松质骨移植材料的孔隙率进行对比研究，为骨组织工程支架材料的研制和开发提供理论依据。方法首先取新鲜尸体的股骨和新鲜猪股骨，进行去除多余组织、灭菌、低温冷冻保存等处理后，制备成松质骨移植材料，使用显微CT分别扫描同种异体骨和动物源性猪骨移植材料获取显微CT图像。然后对同种异体松质骨组织进行染色和切片等处理，制备成同种异体骨组织切片材料，显微镜下观察骨组织切片并获取其图像，各取10幅图像用于实验。设计matlab程序对所有图像进行灰度化、图像增强、二值化、移除干扰等处理，计算孔隙率的数值。结果显微CT和组织切片测试得到的同种异体松质骨孔隙率与动物源性猪松质骨孔隙率结果无显著性差异(P>0.05)。结论显微CT标本的制备较组织切片大为简化，可以无损地获取数据，并省去灰度化、图像增强、移除干扰等处理过程，比组织切片法测量松质骨孔隙率更加简便。

Objective To compare the cancellous bone graft substitute porosity by micro CT image method and biopsy image method, to provide a theoretical basis for scaffold material research and development of bone tissue engineering. Methods First, the femur of a fresh human body and a fresh pig body were taken to remove excess tissue, for sterilization, cryopreservation and other treatments to prepare for cancellous bone graft material, and to obtain the images of allograft bone and animal pig bone graft materials under micro-CT scan. Then by staining and cutting allogeneic cancellous bone tissue, we prepared for bone tissue section material, observed allograft bone tissue sections under a microscope and got 10 images for each section in the three groups. Finally matlab program was designed for grayscale, enhancement, binarization and removing the interference to calculate the value of the porosity. Results The porosity of allogeneic bone and animal pig cancellous bone graft materials that were measured by micro CT method and tissue section imaging method showed no significant differences(P>0.05). Conclusions Compared with tissue section, the preparation of micro CT specimen are simplified, non-destructive and eliminating the processes for grayscale, image enhancement and interference cancellation. Therefore, the micro CT image method for the cancellous bone graft substitute porosity is more convenient than the biopsy image method.

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