北京生物医学工程

DNA电化学传感器中电极修饰条件的探讨_________

Modified conditions of DNA electrochemical sensor

作者：赵燕珍王捷张海燕吴小丽

单位：           南方医科大学(广州510515)    

关键词： DNA电化学传感器；自组装；金电极；循环伏安法

分类号：

出版年·卷·期（页码）：2013·32·2（201-205）

摘要：

目的探讨ssDNA单层膜的制备的最佳条件。方法利用自组装单分子膜技术，将人工合成并修饰的含22个碱基的特异性单链DNA序列固定到金电极上制备成DNA修饰的金电极，通过控制DNA溶液的浓度、作用时间，以及改变修饰基团探讨最佳的修饰条件，并利用循环伏安法初步研究了该修饰电极的电化学行为。结果通过对不同标记探针、不同探针浓度及不同标记时间的表征，结果显示巯基化ssDNA和二硫键修饰的ssDNA探针浓度为80μmol /L,自组装固定时间为12 h时对DNA探针的固定效率最佳。结论巯基化ssDNA和二硫键修饰的ssDNA通过自组装方法可在金电极表面形成稳定、有序的自组装单分子膜(self-assembled monolayer，SAM)，可应用于SAM为基底的DNA传感器的设计与应用。

Objective To investigate the best preparation condition of ssDNA single-layer film. Methods A DNA modified gold electrode was obtained with the technology of self-assembled monolayer(SAM). The concentration of DNA and the time of modification were studied by cyclic voltammetric technique. Results The results showed that a SAM was formed on the surface of gold electrode. The best SAM could be obtained in 80μmol/L capture fixed for 12 h. Conclusions The generation of sulfur gold key could form a stabilized SAM in gold electrodes.

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