Objective Superconducting magnet can generate large gradient high magnetic field (LGHMF) which can simulate different gravity environment and is the primary means of suspension for diamagnetism substance like water，bio-organism and other anti-magnetic materials.In order to accurately locate the sites of anti-mag levitation or other simulated gravity level in superconducting magnet，we established simulated gravity level analysis model of diamagnetism substance (especially for bio-organism) in LGHMF，which should be a great help in magnetobiology and electromagnetic medical field research field.Methods This model calculated magnetic field gradient product and finally got data of diamagnetism substance’s simulated gravity by collecting the data of magnetic intensity and applying central differencing algorithm.Meanwhile this model combined with MATLAB software to fit continuous simulated gravity level function on the magnet longitudinal direction，and make numerical simulation by graphics module of the software.Results This model accurately calculated force analysis of substance and sites of all simulated gravity level in a magnetic field.Simulation could appropriately reflect simulated gravity level trends of diamagnetism substance in magneto-gravitational environment.Conclusions This mathematic model is really important for secondary development of magnet equipment and the design and analysis of magnetic biology experiment.