Objective This paper intends to use coded excitation technique in ophthalmological ultrasound biometer to cut down the emission voltage in order to substitute the traditional single pulse excitation. Methods The Golay complementary sequences were adopted for coded excitation and the echoes were compressed accordingly. By this method,the excitation voltage could be cut down from 60 V to ±15 V. The one-reflector experiment,the resolution measuring experiment and an in vivo experiment were designed in this paper to verify the effectiveness of the method. Results The results showed that when we cut down the excitation voltage by using Golay-coded pulse excitation,we could maintain the same detective depth,signal intensity,resolution and measurement accuracy as the high excitation voltage. Conclusions The coded excitation can reduce the ultrasonic instantaneous emission power and meet the related detection requirements of acoustic output parameters,which has profound significance in the realization of accurate measurement for ophthalmological tissues.