Mechanical stress can regulate cell proliferation,migration,differentiation and other functions. The signal transduction research of intracellular stress mainly focuses on the biochemical signaling pathways,however,the physical delivery process of the stress within the cells has a significant effect on cell function. In this paper,the investigation of mechanotransduction in the intracellular physical delivery process are summarized,including the research on cell membrane,cytoskeleton,endoplasmic reticulum and nucleus. Related technologies including chemical reagent interference,theoretical calculation and fluorescence protein technology are also reviewed to figure out the basic pathways of physical delivery of intracellular stress.