Objective Both basic and applicable research in biomedicine have growing demands in more effective approaches that build direct connections between molecules,cells,systems and bodies.One of the solutions is to establish the remote control of cellular processes via non-contact manners.Optogenetics,as one of the breakthroughs,has caught great attentions in the field.The key issue here is how to implement the remote control of crucial entities and processes at the cell and molecular levels.Therefore,based on mechanical force through magnetics and ultrasonics,assisted with ultrasound microbubble,magnetic nanoparticles and mechanosensitive ion channels,we propose a novel solution to the remotely controlled cells.Methods We used ultrasonic generator to stimulate microbubbles around cells to induce mechanical force for membrane sonoporation.Yellow fluorescent protein (YFP) expression with fluorescence as the index indicated the efficiency of sonoporation thus DNA transported under different conditions.For magnetic control of transmembrane Ca2+,chemically-synthesized magnetic nanoparticles (MNP) were driven by electromagnetic control system.Results Aided with mechanical stimuli,substantial amount of YFP plasmids successfully went across cell membrane and got expressed.The fluorescence of cells co-cultured with MNPs was enhanced upon stimulation of magnetic control system.Conclusions Our results demonstrate that based on the remote control of mechanical force,one can achieve the remote control of transmembrane transport of key biomolecules.The transmembrane transport and expression of DNA can be accomplished through sonoporation (ultrasound strength: >0.6W/cm2).Cell membrane excitability as well as Ca2+ signals could be enhanced through electromagnetics (18 to 20V,>30mT).