Objective At present, there is no objective evaluation on laparoscopic simulation training effect based on hand-eye motion characteristics. The objective of this study is to evaluate basic laparoscopic surgical skill training effect based on Micron Tracker binocular vision navigator and eye tracking system. Methods The tip motion path length of the laparoscopic instrument was calculated, according to the spatial coordinate collected by Micron Tracker binocular vision navigator. Eye motion parameters of trainers were recorded by eye tracking system. Complete time, fixation time, saccadic rate and saccadic amplitude parameters were computed based on eye motion to evaluate training effects. Results With the increase of trials, performance of the trainer was enhanced and become stable. Compared the last trail with the first one: the complete time decreased 58.18%, the right path length decreased 63.95%, the left path length decreased 56.28%, the left path length was always less than the right path length. Saccadic rate decreased 20.81% with the downtrend, saccadic amplitude increased 28.39% with the uptrend.There is no significant difference (P>0.05) in fixation rate and the mean fixation time. Conclusions Hand-eye motion parameters (complete time, instrument motion path length, saccadic rate and saccadic amplitude) can be used as objective indicators to evaluate training effect.